What is the role of DMSO in PCR

PCR - Polymerase Chain Reaction pp 1-8 | Cite as

  • Hans-Joachim Müller
  • Daniel Ruben Prange
Chapter
First Online:

Summary

No other molecular biological technology has shown such a great influence on research into the human genome and the understanding of the molecular mechanisms of genetic diseases as PCR. This method enables the reproduction (amplification) of very small amounts of nucleic acid from a wide variety of starting materials. The PCR links many different disciplines (molecular biology, cell biology, medicine, immunology, diagnostics, forensic medicine, etc.), whereby this technique for examining various questions almost always follows the same procedure.

The in vitro Amplification of DNA (deoxy-ribonucleic acid, also known as DNA “deoxy-ribonucleic acid”) was invented in 1983 by Kary Mullis. He was awarded the Nobel Prize in 1993 for this “invention”. The new technology became known as the polymerase chain reaction, or “PCR” for short, because of the exponential growth rate of DNA. Since the first in 1985 by Saiki et al. published PCR publication, the number of PCR publications increased exponentially.

This is a preview of subscription content, log in to check access.

literature

  1. Lundberg KS et al (1991) High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene 108: 1-6 CrossRefPubMedGoogle Scholar
  2. Motz M et al (2002) Elucidation of an Archaeal Replication Protein Network to Generate Enhanced PCR Enzymes. J Biol Chem 277: 16179-16188 CrossRefPubMedGoogle Scholar
  3. Newton CR, Graham A (1997) PCR. Focus series. Spektrum Akad. Verl., Heidelberg, Berlin, OxfordGoogle Scholar
  4. Saiki RK et al (1985) Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230: 1350-1354 CrossRefPubMedGoogle Scholar
  5. Saiki RK et al (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239 (4839): 487-491 CrossRefPubMed Google Scholar
  6. Sarkar C et al (1990) Formamide can dramatically improve the specificity of PCR. Nucl Acids Res 18 (24): 7465 CrossRefPubMedPubMedCentralGoogle Scholar
  7. Scharf SJ et al (1986) Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science 233 (4768): 1076-8 CrossRefPubMed Google Scholar
  8. Wang Y et al (2004) A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro. Nucleic Acids Res 32 (3): 1197-1207 CrossRefPubMedPubMedCentral Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1. Neckargemünd, Germany
  2. 2. Kiel Germany