What is the role of DMSO in PCR

PCR - Polymerase Chain Reaction pp 1-8 | Cite as

  • Hans-Joachim Müller
  • Daniel Ruben Prange
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No other molecular biological technology has shown such a great influence on research into the human genome and the understanding of the molecular mechanisms of genetic diseases as PCR. This method enables the reproduction (amplification) of very small amounts of nucleic acid from a wide variety of starting materials. The PCR links many different disciplines (molecular biology, cell biology, medicine, immunology, diagnostics, forensic medicine, etc.), whereby this technique for examining various questions almost always follows the same procedure.

The in vitro Amplification of DNA (deoxy-ribonucleic acid, also known as DNA “deoxy-ribonucleic acid”) was invented in 1983 by Kary Mullis. He was awarded the Nobel Prize in 1993 for this “invention”. The new technology became known as the polymerase chain reaction, or “PCR” for short, because of the exponential growth rate of DNA. Since the first in 1985 by Saiki et al. published PCR publication, the number of PCR publications increased exponentially.

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© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1. Neckargemünd, Germany
  2. 2. Kiel Germany